ANALYTICAL CHEMISTRY III AND LABORATORY

The aim of the course is to provide a first framework for the overall synthesis of analytical chemistry, addressing principally the fundamentals and the use of some of the most important instrumental quantitative techniques of analysis and requiring a deep integration of the previously acquired knowledge in the field of analytical chemistry and placed at the end of the study path to exploit the student's maturation.

The course will be carried out through a first part of lectures to provide the student with the basic background for the design of experiments and the use of advanced analytical tools, and a part of the laboratory that provides participated lessons, simulation and the execution of rigorous quantitative analysis with single and group work typology. In the final part of the workshop the students will be invited, under the professor supervision, to create independent working groups which will choose a case study. Each team deal with the analytical problem through the following self-organized work scheme: bibliographic research, feasibility, sampling, choice of analytical method, instrumental analysis, interpretation of the final data and discussion / comparison of their results in the classroom in the presence of the other teams through a multimedia lecture.

Optical methods. Absorption and emission spectra. Absorption and atomic spectroscopy. Atomic spectra. Fundamentals of technique and applications. Instrumentation Diagram. Determination of the content of metals in real matrices. Spectrofluorimetry. Fundamentals of qualitative and quantitative analysis. Conductometry. Voltammetry: characterization of a redox system by cyclic voltammetry. Determination of trace analytes with voltammetric pulsed techniques. Chromatographic methods. Introduction to chromatographic techniques. Van Deemter's equation. Column chromatography and thin layer chromatography. Gas chromatography: determination of alcohols in alcoholic distillates. High Performance Liquid Chromatography. Ion chromatography: determination of the content of cations and anions in drinking water. Mass Spectrometry. Instrumentation Diagram. Ionization Techniques. Separation of charged particles. Magnetic, Quadruple, Ion Trap, Time-flight, and Orbital Trap mass analyzers. Advanced micro-separation techniques CE, ITP, MEKC, LECE.

1. D. A. Skoog, Holler, Crouch, Chimica Analitica Strumentale II edizione, EdiSES, Napoli, 2009.

2. D. C. Harris, Chimica Analitica Quantitativa, III edizione, Zanichelli, 2017.

3. Skoog, West, Holler, Crouch, Fondamenti di Chimica Analitica, III edizione, EdiSES, 2015.

4. Skoog, Holler, Crouch, Principles of instrumental analysis - VII ed. Thomson Brooks/Cole, 2007.

5. D. C. Harris, Quantitative Chemical Analysis, 9th ed. W.H. Freeman and Company, New York, US, 2016.