TECHNIQUES FOR ANALYSIS OF BIOMOLECULES

Learning of the techniques (NMR, CD and XAS) used for the characterization of biomolecules (small molecules, proteins, nucleic acids)

• The physical basis of NMR spectroscopy. Nuclear Angular Moment and magnetic moment. Nuclei in a magnetic field.
• Basic Principles of the NMR experiment. The resonance condition. The pulse. Relaxation. Fourier transformation.
• The Chemical Shift. Nuclear shielding.
• Coupling. Spin-Spin coupling. Equivalent neighboring nuclei. Dependence of bond angle.
• Experimental aspects. The pulse angle. FID. Quadrature detection. Window functions
• Double resonance experiments. Simplification of spectra by selective spin decoupling. NOE. effect. Decoupling in ¹³C NMR spectra.
• NMR 2D. Basic Principles. COSY. TOCSY, ETCOR, NOESY e ROESY. 3D and 4D NMR. Exercises.
• Dynamic NMR. Exchange processes. DOSY.
• MRI. Basic Principles. Contrast agents used in clinic.
• Circular dichroism spectroscopy. Physical basis of CD. Selection rules. Exciton coupling. Static and dynamic coupling. Octant rule. CD sepctra of biomolecules.
• XAS spectroscopy. Physical bases. Application for the study of metalloporteins.

The Basics of NMR by J.P. Hornak Hypertext based NMR course http://www.cis.rit.edu/htbooks/nmr/nmr-main.htm

Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH

Alison Rodger, Bengt Nordén Circular Dichroism and Linear Dichroism Oxford University Press