METODI PER LO STUDIO DI SISTEMI BIOINORGANICI

Knowledge and understanding: Knowledge of techniques for the study of biomolecules and bioinorganic systems.

Applied knowledge and understanding: Ability to apply the acquired knowledge to interpret experimental data and propose characterization techniques of biomolecule.

Making judgments: Ability to critically interpret the data obtained using the techniques discussed in the course.

Communication skills: Acquire language properties in describing the principles and applications of the techniques learned.

Communication skills: Learning Skill: Develop the necessary skills by participating in the lessons and studying the topics and deepen the knowledge acquired in the context of the characterization of biomolecules.

In-room lectures and discussion.

Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

• Circular dichroism spectroscopy. Physical basis of CD. Selection rules. Exciton coupling. Static and dynamic coupling. Octant rule. CD sepctra of biomolecules.
• XAS spectroscopy. Physical bases. Application for the study of metalloproteins.
• The physical basis of NMR spectroscopy. Nuclear Angular Moment and magnetic moment. Nuclei in a magnetic field.
• Basic Principles of the NMR experiment. The resonance condition. The pulse. Relaxation. Fourier transformation.
• The Chemical Shift. Nuclear shielding.
• Coupling. Spin-Spin coupling. Equivalent neighboring nuclei. Dependence of bond angle.
• Double resonance experiments. Simplification of spectra by selective spin decoupling. NOE. effect. Decoupling in ¹³C NMR spectra.
• NMR 2D. Basic Principles. COSY. TOCSY, ETCOR, NOESY e ROESY. 3D and 4D NMR. Exercises.
• Dynamic NMR. Exchange processes. DOSY.
• MRI. Basic Principles. Contrast agents used in the clinic.
• EPR. Physical bases. Application for the study of metalloproteins.

The Basics of NMR by J.P. Hornak Hypertext based NMR course http://www.cis.rit.edu/htbooks/nmr/nmr-main.htm

Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH

Alison Rodger, Bengt Nordén Circular Dichroism and Linear Dichroism Oxford University Press